Peptide Blend Calculator

How to use the peptide blend calculator

A blend is a single vial that holds two or more peptides freeze-dried together. After you add water, the peptides share the same liquid, so you cannot draw one without the others. The calculator handles that for you in five steps.

1. List every peptide and its mg. Use the per-peptide amounts from the label. Tap a preset to fill common compositions, then check each number against your vial. Add up to four rows.
2. Enter the water. Type the mL of bacteriostatic water you add. Tap a preset volume to compare options before you mix.
3. Say how your amount is written. Choose Dose by one component when your notes give an amount for one peptide, such as 250 mcg of BPC-157. Choose Dose by total blend when the amount covers everything in the vial combined.
4. Enter the amount and pick the syringe. The amount is an arithmetic input only. Choose 0.3, 0.5 or 1 mL. Tick U-40 only if the syringe says U-40.
5. Read the draw and the table. The big number is the unit mark. The table shows each peptide’s concentration and how much of it that draw holds. Show the math lists every step.

Is “10 mg” per peptide or total?

This is the most common blend error, and no calculator can fix it for you. A vial labeled “BPC-157/TB-500 10 mg” can mean two very different things: 5 mg of each peptide (10 mg total), or 10 mg of each (20 mg total). Labels are not standardized, so look for a per-peptide breakdown on the vial, the box or the certificate of analysis (COA), the lab report that lists what a batch contains.

The table below shows why it matters. It uses 2 mL of water and an example amount of 250 mcg of BPC-157 for each reading of the same label.

If the vial really holds 5 mg of each but you calculate as if it holds 10 mg of each, you draw 5 units and get 125 mcg of BPC-157 instead of 250 mcg: half. If the vial really holds 10 mg of each and you assume 5 mg, you draw 10 units and get 500 mcg: double. The calculator shows a Per peptide or total? note whenever the rows are equal, because that is when the mix-up is most likely.

How one draw from a blend splits into its peptides

The diagram follows a GLOW vial mixed with 2 mL of water. The total concentration is 35 mg/mL, but that single number hides three different concentrations, one per peptide.

Peptide blend formulas

A blend is several ordinary reconstitution problems that share one volume of water. Work each peptide out separately, then link them through the volume you draw.

Worked examples

Every amount below is an example input chosen to show the arithmetic. None is a suggested dose.

Example 1: BPC-157 5 mg + TB-500 5 mg, 2 mL, 250 mcg of BPC-157

BPC-157 concentration is 5 × 1000 ÷ 2 = 2,500 mcg/mL. The volume is 250 ÷ 2,500 = 0.1 mL, or 10 units. TB-500 has the same concentration, so the draw also holds 250 mcg of TB-500, 500 mcg of blend in all. The vial holds 20 such draws.

Example 2: the same vial, 250 mcg written as total blend

The total concentration is 5,000 mcg/mL, so 250 mcg of blend is 0.05 mL, or 5 units. That draw holds only 125 mcg of each peptide. Reading a total amount as a per-peptide one, or the reverse, is a two-fold error in a 1:1 blend.

Example 3: GLOW in 3 mL, 500 mcg of BPC-157

BPC-157 is 10 mg of the vial, so its concentration is 3,333.3 mcg/mL. 500 mcg is 0.15 mL, or 15 units, which fits a 0.5 mL syringe. The same draw carries 2.5 mg of GHK-Cu and 500 mcg of TB-500, 3.5 mg in total. The vial holds 20 draws.

Example 4: CJC-1295 (no DAC) 5 mg + ipamorelin 5 mg, 2.5 mL, 100 mcg of CJC-1295

Each peptide is 2,000 mcg/mL. 100 mcg is 0.05 mL, or 5 units, with 100 mcg of ipamorelin alongside. A draw this small is easier to read on a 0.3 mL syringe, which is often marked in half units. The vial holds 50 draws.

Example 5: KLOW in 2 mL, 4 mg written as total blend

80 mg in 2 mL is 40 mg/mL in total, so 4 mg of blend is 0.1 mL, or 10 units. It splits into 2.5 mg GHK-Cu, 500 mcg BPC-157, 500 mcg TB-500, 500 mcg KPV.

Blend charts: what 10 units contains

These charts show how much of each peptide sits in 10 units (0.1 mL) of a U-100 syringe for the preset compositions. Divide an amount by the “per 10 units” value and multiply by 10 to get units. For any other volume or composition, use the calculator above.

In a 1:1 blend, each peptide is exactly half of the blend total in any draw.

The same amount of one peptide can mean very different draws, and very different company, depending on the blend. Here is an example amount of 250 mcg of BPC-157 at 2 mL of water in each BPC-157 preset:

GLOW and KLOW blends: what the labels usually say

GLOW and KLOW are informal names used by research-peptide sellers, not drug names. The compositions below are the ones most often printed on labels. Products differ, so treat the presets as a starting point and always enter your own vial’s numbers.

• GLOW: GHK-Cu 50 mg + BPC-157 10 mg + TB-500 10 mg, 70 mg in total. GHK-Cu is a copper-binding tripeptide (glycyl-histidyl-lysine).
• KLOW: the GLOW trio plus KPV 10 mg, 80 mg in total. KPV is a three-amino-acid peptide (lysine-proline-valine) from the tail end of alpha-MSH, a natural hormone.

The practical point is the ratio. GHK-Cu is 71.4% of GLOW and 62.5% of KLOW by mass. So 1 mg of GLOW blend holds 714.29 mcg of GHK-Cu, 142.86 mcg of BPC-157, 142.86 mcg of TB-500. If your notes give an amount of BPC-157 and you enter it as a total-blend amount, the draw holds only one-seventh of that BPC-157. Use Dose by one component for that case. The BPC-157 calculator gives the same answer as a blend only if you enter the blend’s BPC-157 mg (10 mg in GLOW), not the vial total.

CJC-1295 and ipamorelin blend reconstitution

The usual label is CJC-1295 (no DAC) 5 mg + ipamorelin 5 mg in one 10 mg vial. “No DAC” means the version without the drug affinity complex, a chemical add-on that binds the peptide to albumin in the blood and makes it last much longer. The two versions are different substances, so check which one the label names.

Because the ratio is 1:1, the math is simple. With 2 mL of water, each peptide is 2.5 mg/mL and every U-100 unit holds 25 mcg of CJC-1295 and 25 mcg of ipamorelin. Divide an amount of either peptide by 25 to get units. If your notes give a combined amount, halve it first, or select Dose by total blend. To choose a water volume that lands your amount on a whole mark, use the BAC water calculator with the per-peptide mg (5 mg), not the vial total.

Regulatory and anti-doping status

None of the peptides in these presets is an FDA-approved drug. Their compounding status has changed several times since 2023, so here is the timeline from FDA’s own documents.

• September 29, 2023: FDA placed BPC-157, TB-500 (listed as thymosin beta-4 fragment LKKTETQ), CJC-1295, ipamorelin acetate, KPV and injectable GHK-Cu in Category 2 of its 503A bulk substances list: substances that raise significant safety concerns. For BPC-157, FDA cited a possible risk of immunogenicity (an immune reaction to the peptide) for certain routes of administration, and problems with peptide-related impurities and characterizing the active ingredient.
• April 2026: the nominators withdrew those nominations, and FDA moved the peptides to a list of substances “nominated but withdrawn.” FDA’s page still describes the same safety risks for each. Ipamorelin acetate remains in Category 2 for 503B outsourcing facilities.
• July 23, 2026: FDA’s Pharmacy Compounding Advisory Committee (PCAC) reviewed BPC-157, KPV, TB-500 and MOTs-C for the 503A bulks list. It voted 8–6, with one abstention, to recommend adding BPC-157, against the advice of FDA staff. The vote is non-binding. FDA makes the final decision, and a compounding listing would not be a drug approval.

For athletes, the rules are clearer. The WADA 2026 Prohibited List, in force from January 1, 2026, names BPC-157 under S0 (non-approved substances), CJC-1295 and ipamorelin under S2.2.4 (growth hormone releasing factors), and thymosin beta-4 derivatives such as TB-500 under S2.3 (growth factors). All are prohibited at all times, in and out of competition. GHK-Cu and KPV are not named, but S0 covers any substance with no current government approval for human therapeutic use, so check with your anti-doping organization.

Common blend mistakes

Each mistake below maps to a check in the calculator, so a warning appears before the number is used.

• Treating a total label as per peptide. A “10 mg” blend that is 5 + 5 entered as 10 + 10 halves the real amount in every draw. The Per peptide or total? note flags equal rows.
• Treating a total-blend amount as one peptide. 500 mcg of a 1:1 blend is 250 mcg of each. The Total, not per peptide note spells out the split.
• Typing the vial amount into the dose box. If one draw would use half the vial or more, the calculator asks whether you entered the label amount by mistake.
• Using a single-peptide calculator on a blend. Entering the vial total into the reconstitution calculator gives the blend’s total concentration, not any one peptide’s.
• Mixing up mg and mcg. 1 mg is 1,000 mcg. The mcg to mg converter helps when labels and notes use different units.
• Using a U-40 syringe. Each U-40 unit is 2.5 times the volume of a U-100 unit. The mg to units calculator explains the difference.
• Forgetting the vial runs out for every peptide at once. The doses per vial calculator shows how long a vial lasts at your schedule.

Key terms

Blend

One vial containing two or more peptides freeze-dried together and reconstituted in the same water.

Component

One peptide in a blend, such as BPC-157 in GLOW.

Ratio

The fixed proportion between components, such as 5 : 1 : 1 in GLOW. Adding water never changes it.

Total concentration

All components combined per mL. Useful only when your amount is written for the whole blend.

Certificate of analysis (COA)

A lab report listing what a batch contains, often including the per-peptide amounts.

503A bulks list

FDA’s list of bulk drug substances that state-licensed pharmacies may use to compound for individual prescriptions.

WADA S0

The Prohibited List class for substances with no government approval for human therapeutic use.

Frequently asked questions

Sources

We check every formula and figure on this page against primary sources. See our editorial policy for how we review content.